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Related Experiment Videos

Sequence and domain structure of talin.

D J Rees1, S E Ades, S J Singer

  • 1Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.

Nature
|October 18, 1990
PubMed
Summary

Talin, a cytoskeletal protein, interacts with integrins at cell adhesion sites. This study reveals talin

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Area of Science:

  • Cell biology
  • Molecular biology
  • Biochemistry

Background:

  • Talin is a high-molecular-weight cytoskeletal protein found at cell adhesion points.
  • Integrins mediate cell attachment to extracellular matrices and other cells.
  • Talin codistributes with integrins at the cell surface membrane during adhesion.

Purpose of the Study:

  • To investigate the structure of talin.
  • To understand talin's role in transmembrane connections between extracellular adhesions and the cytoskeleton.

Main Methods:

  • Cloning and sequencing of murine talin.
  • Analysis of talin sequence to propose a structural model.
  • In vitro binding studies to assess protein interactions.

Main Results:

  • Talin binds to integrins with low affinity and to vinculin with high affinity.
  • Talin is a substrate for calpain II, a calcium-activated protease.
  • A structural model for talin was proposed based on sequence data.

Conclusions:

  • Talin plays a crucial role in linking the cytoskeleton to the plasma membrane.
  • Sequence homologies suggest talin belongs to a novel family of submembranous cytoskeleton-associated proteins.
  • These proteins are involved in connections to the plasma membrane.

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