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Related Experiment Video

Updated: Jun 5, 2026

Two-Photon-Based Photoactivation in Live Zebrafish Embryos
09:10

Two-Photon-Based Photoactivation in Live Zebrafish Embryos

Published on: December 24, 2010

Two-photon-based photoactivation in live zebrafish embryos.

Niva Russek-Blum1, Helit Nabel-Rosen, Gil Levkowitz

  • 1Molecular Cell Biology, Weizmann Institute of Science.

Journal of Visualized Experiments : Jove
|January 6, 2011
PubMed
Summary

Researchers used two-photon microscopy to activate caged fluorescein in zebrafish embryos, enabling precise tracking of cell development. This method allows in vivo investigation of biological processes at a single-cell level.

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Area of Science:

  • Developmental Biology
  • Microscopy Techniques
  • Chemical Biology

Background:

  • Photoactivation is crucial for studying biological processes in vivo.
  • Multi-photon microscopy allows precise light activation in deep tissues.
  • Zebrafish embryos offer optical transparency for in vivo monitoring.

Purpose of the Study:

  • To demonstrate a method for precisely activating caged compounds in live zebrafish embryos.
  • To track cell fate during embryonic development using light-induced activation.
  • To establish a versatile technique for studying biological processes with spatiotemporal control.

Main Methods:

  • Utilizing two-photon microscopy for targeted photoactivation.
  • Employing caged fluorescein as a model light-responsive agent.

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Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos
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Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos

Published on: February 16, 2009

Single Cell Fate Mapping in Zebrafish
07:53

Single Cell Fate Mapping in Zebrafish

Published on: October 5, 2011

Related Experiment Videos

Last Updated: Jun 5, 2026

Two-Photon-Based Photoactivation in Live Zebrafish Embryos
09:10

Two-Photon-Based Photoactivation in Live Zebrafish Embryos

Published on: December 24, 2010

Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos
12:21

Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos

Published on: February 16, 2009

Single Cell Fate Mapping in Zebrafish
07:53

Single Cell Fate Mapping in Zebrafish

Published on: October 5, 2011

  • Using zebrafish embryos expressing green fluorescent protein (GFP) as genetic landmarks.
  • Monitoring cell fate in vivo with single-cell resolution.

    • Main Results:

    • Successfully photoactivated caged fluorescein within zebrafish embryos.
    • Demonstrated the ability to track the fate of activated cells in live embryos.
    • Validated the precision of two-photon microscopy for targeting specific cells.

    Conclusions:

    • Two-photon microscopy enables precise photoactivation of caged compounds in zebrafish embryos.
    • This technique facilitates in vivo tracking of cell destiny and biological processes.
    • The method is adaptable for activating various light-sensitive molecules and proteins.