Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bacillus subtilis ada operon encodes two DNA alkyltransferases.

F Morohoshi1, K Hayashi, N Munakata

  • 1Radiobiology Division, National Cancer Center Research Institute, Tokyo, Japan.

Nucleic Acids Research
|September 25, 1990
PubMed
Summary

Bacillus subtilis DNA fragments were identified that repair mutations caused by MNNG. The adaA gene activates repair, while adaB repairs O6-methylguanine DNA damage, revealing insights into DNA repair mechanisms.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structure and expression pattern of a human MTG8/ETO family gene, MTGR1.

Gene·2000
Same author

Base substitution spectra of nalidixylate resistant mutations induced by monochromatic soft X and 60Co gamma-rays in Bacillus subtilis spores.

Journal of radiation research·1999
Same author

Genomic structure of the human RBP56/hTAFII68 and FUS/TLS genes.

Gene·1998
Same author

The AML1-MTG8 leukemic fusion protein forms a complex with a novel member of the MTG8(ETO/CDR) family, MTGR1.

Molecular and cellular biology·1998
Same author

Induction of unique tandem-base change mutations in bacterial spores exposed to extreme dryness.

Mutation research·1997
Same author

Cloning and mapping of a human RBP56 gene encoding a putative RNA binding protein similar to FUS/TLS and EWS proteins.

Genomics·1996

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • The adaptive response is crucial for bacterial survival against DNA alkylating agents.
  • Bacillus subtilis mutants (ada) show hypersensitivity to N-methyl-N'-nitro-N-nitrosoguanidine (MNNG).

Purpose of the Study:

  • To identify and characterize Bacillus subtilis DNA fragments that complement MNNG hypersensitivity in ada mutants.
  • To elucidate the function of genes involved in DNA repair and adaptive response.

Main Methods:

  • Prophage transformation and subcloning were used to isolate functional DNA fragments.
  • Nucleotide sequencing identified open reading frames (ORFs) for adaA and adaB genes.
  • Transcript analysis using Northern blotting detected specific mRNA transcripts.

Related Experiment Videos

Main Results:

  • Two overlapping genes, adaA and adaB, were identified, encoding methylphosphotriester-DNA methyltransferase and O6-methylguanine-DNA methyltransferase, respectively.
  • The ada operon produced 1.6 Kb transcripts in MNNG-treated cells.
  • adaA acts as a transcriptional activator, and adaB is responsible for repairing O6-methylguanine residues.

Conclusions:

  • The Bacillus subtilis ada operon comprises two genes with distinct roles in DNA repair.
  • The organization differs from Escherichia coli, suggesting gene fusion or splitting during evolution.
  • These findings enhance understanding of bacterial adaptive response mechanisms.