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Related Concept Videos

Introduction to Metabolism01:30

Introduction to Metabolism

Metabolism encompasses all biochemical reactions in a living organism, facilitating both the breakdown and synthesis of biomolecules. These metabolic processes are categorized into catabolic and anabolic pathways, which operate in a coordinated manner to ensure energy balance and cellular function.Catabolic Pathways and Energy ReleaseCatabolic pathways involve the breakdown of complex macromolecules such as carbohydrates, lipids, and proteins into smaller structures like monosaccharides, fatty...
Overview of Metabolism01:40

Overview of Metabolism

Living cells constantly carry out various chemical reactions which are necessary for their proper functioning. These reactions are interlinked to one another via multiple pathways. The collection of these chemical reactions is known as metabolism.
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...

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Related Experiment Video

Updated: Jun 5, 2026

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)

Published on: March 14, 2013

Origins of metabolic profiling.

Arthur B Robinson1, Noah E Robinson

  • 1Oregon Institute of Science and Medicine, Oregon, OR, USA. art@oism.org

Methods in Molecular Biology (Clifton, N.J.)
|January 6, 2011
PubMed
Summary

Quantitative metabolic profiling, a decade-long project, established that analyzing numerous metabolites reveals human health status. This method identified distinct metabolic profiles for diseases and individual variations.

Area of Science:

  • Biochemistry
  • Medical Diagnostics
  • Computational Biology

Background:

  • Quantitative metabolic profiling emerged from a 1968-1978 California research initiative.
  • The study aimed to establish metabolite analysis as a tool for assessing human health.

Purpose of the Study:

  • To demonstrate the utility of quantitative metabolic profiling for measuring human health.
  • To identify diagnostic metabolic profiles for diseases and variations like age, diet, and sex.
  • To confirm the existence of unique, genetically determined metabolic profiles in individuals.

Main Methods:

  • Employed chromatographic and mass spectrometric techniques.
  • Analyzed 50-200 metabolites across over 15,000 human specimens.
  • Utilized computerized pattern recognition for data evaluation.

More Related Videos

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
11:25

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

Published on: July 11, 2014

Related Experiment Videos

Last Updated: Jun 5, 2026

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)

Published on: March 14, 2013

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
11:25

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

Published on: July 11, 2014

Main Results:

  • Achieved statistically significant and diagnostically valuable metabolic profiles.
  • Demonstrated profiles associated with various human diseases.
  • Identified metabolic variations linked to age, diet, fasting, sex, and other factors.
  • Confirmed genetically distinct metabolic profiles in both infants and adults.

Conclusions:

  • Quantitative metabolic profiling is a viable method for assessing human health.
  • The project addressed and largely resolved practical and conceptual challenges in metabolite analysis and application.
  • Individualized metabolic profiling holds potential for medical diagnostics.