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Analysis of the citric acid cycle intermediates using gas chromatography-mass spectrometry

Rajan S Kombu1, Henri Brunengraber, Michelle A Puchowicz

  • 1Department of Nutrition, Mouse Metabolic Phenotyping Center, Case Western Reserve University, Cleveland, OH, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 6, 2011
PubMed

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Gas Chromatography–Mass Spectrometry (GC–MS)

Gas chromatography–mass spectrometry (GC–MS) is the combination of analytical techniques of gas chromatography and mass spectrometry in a single instrument for analyzing a mixture of compounds. The gas chromatograph separates the compounds in the mixture, and the mass spectrometer analyzes each compound separately to determine the molecular masses and molecular structures.
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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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