Related Experiment Video
Updated: Jun 5, 2026

Sample Preparation for Rapid Lipid Analysis in Drosophila Brain Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging
Published on: July 14, 2022
Liquid chromatography-mass spectrometry (LC-MS)-based lipidomics for studies of body fluids and tissues
Heli Nygren1, Tuulikki Seppänen-Laakso, Sandra Castillo
1VTT Technical Research Centre of Finland, Espoo, Finland. heli.nygren@vtt.fi
Abstract:
In this paper, analytical methodologies for the global profiling of lipids in serum and tissue samples are reported. The sample preparation is based on a modified Folch extraction, and the analysis is carried out with ultrahigh-performance liquid chromatography combined with mass spectrometry (UPLC-MS). For further identification, MS(n) mass spectrometry is carried out utilizing an LTQ-Orbitrap mass spectrometry as the detector. Such a system affords determination of accurate masses and is thus a highly useful tool for lipid identification. The repeatability of the analysis proved to be good, with relative standard errors for spiked samples being between 4.51 and 10.44%. The throughput of the methodology described here is over 100 samples a day.
Related Concept Videos
MALDI-TOF Mass Spectrometry
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Matrix-Assisted Laser Desorption Ionization (MALDI)

