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Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases
Published on: December 26, 2011
Identification of nucleohistones by glycosylation and basic dyes
K Jobst1, A Lakatos, A Horváth
1Department of Clinical Chemistry and Pathology, Medical University, Pécs, Hungary.
Acta Histochemica
|January 1, 1990
Abstract:
Earlier examinations have shown that reducing sugars mainly react with the epsilon-amino group of lysine. After DNA extraction, lysine-rich histone proteins of alcohol fixed nuclei of rat organs were treated with glucose-6-phosphate. It was possible to selectively stain the histone type proteins of DNA negative nuclei with basic dyes.

