The synthesis of double-headed nucleosides by the CuAAC reaction and their effect in secondary nucleic acid
Anna S Jørgensen1, Khalil I Shaikh, Gerald Enderlin
1Nucleic Acid Center and Department of Physics and Chemistry, University of Southern Denmark, 5230 Odense M, Denmark.
Abstract:
Four double-headed nucleosides were prepared by the CuAAC reaction. Hereby, a triazole-containing linker connects an additional thymine or adenine to the 2'-position of 2'-deoxyuridine, a thymine to the 5'-position of thymidine and a thymine to the 6'-position of an LNA-thymidine monomer. Whereas no conclusive recognition effects of the additional thymines were found when introduced in LNA or at the 5'-position, both thymine and adenine in the 2'-position were found to stabilise three-way junctions in both dsDNA and DNA : RNA contexts and to give cross-strand interactions in a DNA-duplex, when specifically introduced in a so-called (+1)-zipper motif.
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