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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

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Related Experiment Video

Updated: Jun 5, 2026

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

Alternative splicing enriched cDNA libraries identify breast cancer-associated transcripts.

Elisa N Ferreira1, Maria C R Rangel, Pedro F Galante

  • 1Laboratory of Genomics and Molecular Biology, Hospital A.C. Camargo, Fundação Antonio Prudente, São Paulo, 01509-900, Brazil.

BMC Genomics
|January 8, 2011
PubMed
Summary

This study developed a new method to identify alternative splicing (AS) variants in breast cancer cells. The approach revealed novel AS events and transcripts modulated by ERBB2, offering potential new biomarkers for breast cancer.

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Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

Area of Science:

  • Molecular Biology
  • Genomics
  • Cancer Research

Background:

  • Alternative splicing (AS) generates transcriptome diversity and is implicated in human diseases.
  • Cancer-associated transcripts from AS can serve as diagnostic, prognostic, and therapeutic targets.
  • Exploring AS variability is crucial for understanding cancer complexity.

Purpose of the Study:

  • To develop a transcriptome-wide method for exploring the alternative splicing repertoire.
  • To identify AS variants in breast tumor cells modulated by ERBB2 (HER-2/neu) oncogene expression.
  • To discover novel molecular markers for breast cancer diagnosis and therapy.

Main Methods:

  • Developed a novel approach combining heteroduplex trapping and RNA amplification for AS-enriched library construction.
  • Generated and analyzed 2,048 partial cDNA sequences, identifying 214 AS-enriched tags (ASSETs).
  • Validated AS events using RT-PCR and evaluated ERBB2-mediated AS regulation in mammary cell lines.

Main Results:

  • Identified 138 different AS events from 79 multiple exon ASSETs, with a 94.5% RT-PCR validation success rate.
  • Discovered 2 novel AS events.
  • Demonstrated that ERBB2 expression differentially modulates AS variants in key genes within a model system.

Conclusions:

  • Presented a versatile, transcriptome-wide method for AS exploration adaptable to next-generation sequencing.
  • Identified ERBB2-modulated AS transcripts as potential molecular markers for breast cancer.
  • Highlighted the methodology's utility in deciphering cancer transcriptome diversity and discovering precise molecular markers.