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Updated: Jun 5, 2026

Agarose-Based Model Ecosystem for Cultivating Methanotrophs in a Methane-Oxygen Counter Gradient
Published on: September 6, 2024
Influence of support material properties on the potential selection of Archaea during initial adhesion of a
Frédéric Habouzit1, Gaëlle Gévaudan, Jérôme Hamelin
1INRA, UR50, Laboratoire de Biotechnologie de l'Environnement, Avenue des Etangs, Narbonne F-11100, France. habouzit@supagro.inra.fr
Abstract:
In anaerobic wastewater treatment systems, the complex microbial biomass including Archaea and Bacteria can be retained as a biofilm attached to solid supports. The aim of this study was to evaluate the impact of specific properties of support material on early microbial adhesion. Seven different substrata are described in terms of topography and surface energy. Adhesion of a methanogenic consortium to these substrata was tested, the adhesion was quantified as a percentage of the surface area covered and the bacterial and archaeal community structures was assessed by molecular fingerprinting profiles (CE-SSCP). As expected, the overall adhesion on the supports was influenced mainly by total surface energy. Moreover, the adhered communities were different from the parent inocula, including the Archaea/Bacteria ratio. This could have a significant impact on the start-up of anaerobic digesters for which supports favoring Archaea adhesion, responsible for the limiting reaction of the process, should be preferred.
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