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Updated: Jun 5, 2026

Protein Purification-free Method of Binding Affinity Determination by Microscale Thermophoresis
Published on: August 15, 2013
[Determination of plasma protein binding rate of tetramethylpyrazine phosphate by ultrafiltration]
Yong Ye1, Li-ling Zhou, Yi-lin Yan
1Guangzhou University of Chinese Medicine, China. yong-ye@163.com
Objective:
To study the plasma protein binding rate of Tetramethylpyrazine Phosphate.
Methods:
The ultrafiltration was employed to determine the plasma protein binding rate of Tetramethylpyrazine Phosphate. The plasma concentrations of Tetramethylpyrazine Phosphate were measured by RP-HPLC.
Results:
The plasma protein binding rate of Tetramethylpyrazine Phosphate with HSA, human plasma and rat plasma were (64.64 +/- 0.68)%, (65.85 +/- 7.35)% and (73.65 +/- 2.35)%, respectively.
Conclusion:
The binding rate of Tetramethylpyrazine Phosphate with plasma protein is middling strength.
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