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Control of c-Jun activity by interaction of a cell-specific inhibitor with regulatory domain delta: differences

V R Baichwal1, R Tjian

  • 1Howard Hughes Medical Institute, University of California, Berkeley 94720.

Cell
|November 16, 1990
PubMed

Insights

Cellular inhibitors negatively regulate c-Jun transcriptional activity via the A1 domain, with the delta domain mediating this repression. This explains why v-Jun, lacking delta, is a stronger activator than c-Jun.

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Cellular Signaling

Background:

  • c-Jun is a transcription factor involved in various cellular processes.
  • Transcriptional activity of c-Jun can be modulated by regulatory domains.
  • Differences in transcriptional properties exist between c-Jun and its viral counterpart, v-Jun.

Purpose of the Study:

  • To investigate the transcriptional activation properties of c-Jun.
  • To identify the regulatory mechanisms controlling c-Jun activity in vivo.
  • To elucidate the role of the delta domain in c-Jun regulation.

Main Methods:

  • Analysis of c-Jun chimeras in different cell lines.
  • In vitro transcription assays using chimeric Jun proteins and cell extracts.
  • Investigating the interaction between regulatory domains and cellular inhibitors.

Main Results:

  • c-Jun possesses an activator domain (A1) negatively regulated by a cell type-specific inhibitor.
  • The delta domain of c-Jun facilitates the interaction between the inhibitor and A1.
  • v-Jun, lacking the delta domain, exhibits stronger transcriptional activation due to reduced repression.
  • Both A1 and delta domains are subject to cell type-specific repression.

Conclusions:

  • A specific cellular factor negatively regulates c-Jun activity.
  • The delta domain plays a crucial role in mediating cell type-specific repression of c-Jun.
  • The findings provide a molecular explanation for the distinct transcriptional behaviors of c-Jun and v-Jun.

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