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Updated: Jun 5, 2026

A Microfluidic Chip for ICPMS Sample Introduction
Published on: March 5, 2015
Monolithic integration of two-dimensional liquid chromatography-capillary electrophoresis and electrospray ionization
Andrew G Chambers1, J Scott Mellors, W Hampton Henley
1Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599, USA.
Abstract:
A microfluidic device capable of two-dimensional reversed-phase liquid chromatography-capillary electrophoresis with integrated electrospray ionization (LC-CE-ESI) for mass spectrometry (MS)-based proteomic applications is described. Traditional instrumentation was used for the LC sample injection and delivery of the LC mobile phase. The glass microfabricated device incorporated a sample-trapping region and an LC channel packed with reversed-phase particles. Rapid electrokinetic injections of the LC effluent into the CE dimension were performed at a cross-channel intersection. The CE separation channel terminated at a corner of the square device, which functioned as an integrated electrospray tip. In addition to LC-CE-ESI, this device was used for LC-ESI without any instrumental modifications. To evaluate the system, LC-MS and LC-CE-MS analyses of protein digests were performed and compared.
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