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Updated: Jun 5, 2026
Isolation of Murine Embryonic Hemogenic Endothelial Cells
Published on: June 17, 2016
[FLK-1+ cells derived from mouse fetal liver could differentiate into endothelial cells and smooth muscle cells]
Cheng-Hao Jin1, Yoshikazu Yonemitsu, Wei-Rong Ding
1Department of Hematology, Jiangxi Provincial People's Hospital, Nanchang 330006, China. jinch227@yahoo.com.cn
Objective:
To characterize the differential and proliferative activities of FLK-1(+) cells derived from mouse fetal liver.
Method:
The FLK-1(+) fraction were enriched from the fetal liver using immunomagnetic method and their differential and proliferative activities were examined in culture medium and in vivo via transplantation of FLK-1(+) cells into the inferior pole of the spleen of nine-week-old male C57 BL/6 mice after two-thirds hepatectomy.
Results:
In response to growth factors, FLK-1(+) cells expressed typical lineage-specific markers for vascular endothelial cells, smooth muscle cells. Intrahepatic implantation of FLK-1(+) cells resulted in the formation of blood vessels in the fibrous capsule of partially hepatectomized liver.
Conclusion:
These results indicate that FLK-1(+) cells derived from mouse fetal liver could differentiate into endothelial cells and smooth muscle cells and they may serve as potential stem cells for clinical cell-based therapy.
