[Study on the induced differentiation of bone marrow-derived mesenchymal stem cells into inner ear hair cell-like cells in vitro]
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Updated: Jun 5, 2026
Initiating Differentiation in Immortalized Multipotent Otic Progenitor Cells
Published on: January 2, 2016
Yong-ming Yang1, Hong-qun Jiang
1Department of Otorhinolaryngology Head and Neck Surgery, First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
Objective:
To investigate the feasibility of orientation-induced differentiation of rat bone marrow-derived mesenchymal stem cells (MSC) into cochlear hair cell-like cells in vitro.
Methods:
MSC were extracted, cultured and identified. The organ of Corti was isolated from post natal day 1-day 3 Sprague-Dawley rats. Then MSC were co-cultured with organ of Corti for 14 days in vitro. The expression of hair cell markers (myosin VIIa, math1 and calretinin) was detected by immunocytochemical analyses and by reverse transcription-polymerase chain reaction (RT-PCR).
Results:
The differentiated cells were immunopositive for myosin VIIa, calretinin and math1. And RT-PCR results indicated that these differentiated cells expressed hair cell markers Math1 and myosin VIIa.
Conclusions:
MSC cultured in vitro could be successfully induced to differentiate into hair cell-like cells with hair cell molecular markers.
