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Updated: Jun 5, 2026

Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis
Published on: September 7, 2021
Blastocyst culture is associated with an elevated incidence of monozygotic twinning after single embryo transfer
Satoshi Kawachiya1, Daniel Bodri, Naoko Shimada
1Kato Ladies Clinic, Tokyo, Japan. s-kawachiya@towako-kato.com
Abstract:
In a 7-year (2002-2008) retrospective study of a large IVF program based on minimal ovarian stimulation and single ET (47,841 single ETs), monozygotic twinning occurred in 1.01% of 14,956 clinical pregnancies. Blastocyst culture was associated with a significantly increased monozygotic twinning risk (adjusted odds ratio, 2.04; 95% confidence interval, 1.29-4.48), whereas embryo freezing, type of stimulation protocol used, intracytoplasmic sperm injection fertilization, or zona removal did not influence its incidence.
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