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Related Experiment Video

Updated: Jun 5, 2026

Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes
09:28

Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes

Published on: January 10, 2017

Interactions between single-walled carbon nanotubes and lysozyme.

F Bomboi1, A Bonincontro, C La Mesa

  • 1Physics Dept., La Sapienza University, Rome, Italy.

Journal of Colloid and Interface Science
|January 11, 2011
PubMed
Summary

This study explores how pH and lysozyme concentration affect carbon nanotube stability in water. Higher protein concentrations initially stabilize nanotubes, but excessive amounts cause aggregation due to depletion effects.

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Last Updated: Jun 5, 2026

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Published on: January 10, 2017

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Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube (SWCNT)-delivered MALAT1 Antisense Oligos

Published on: December 13, 2018

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Physical Chemistry

Background:

  • Carbon nanotubes (CNTs) are nanomaterials with unique properties but often aggregate in solution.
  • Stabilizing CNTs in aqueous media is crucial for their application in various fields.
  • Proteins like lysozyme can act as stabilizers for nanomaterials.

Purpose of the Study:

  • To investigate the stabilizing effect of lysozyme on single-walled carbon nanotube (SWCNT) dispersions.
  • To understand the influence of pH and protein concentration on SWCNT-lysozyme interactions.
  • To elucidate the mechanisms of stabilization and destabilization in these systems.

Main Methods:

  • Electrophoretic mobility measurements to assess surface charge.
  • Dielectric relaxation spectroscopy to probe the electrical double layer.
  • Circular dichroism to evaluate protein conformation.
  • Varying pH and lysozyme concentrations to observe dispersion behavior.

Main Results:

  • Lysozyme concentration and pH significantly modulate SWCNT-protein interactions and dispersion stability.
  • Complex formation between lysozyme and SWCNTs was observed, with binding indicated by surface charge and double layer changes.
  • Above a critical protein/nanotube mass ratio, depletion effects led to SWCNT aggregation and phase separation.

Conclusions:

  • Lysozyme can stabilize SWCNTs in aqueous solutions, with stability dependent on pH and protein concentration.
  • The binding of lysozyme to SWCNTs appears to preserve its native conformation.
  • Protein-based depletion phenomena can counteract stabilization, leading to aggregation, similar to surfactant systems.