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IgG and IgM antibodies to the refolded MOG(1-125) extracellular domain in humans

Francesca Gori1, Barbara Mulinacci, Lara Massai

  • 1Laboratorio Interdipartimentale di Chimica e Biologia di Peptidi e Proteine, Dipartimento di Scienze Farmaceutiche, Università degli Studi di Firenze, Via Ugo Schiff 6, 50019, Sesto Fiorentino, Firenze, Italy.

Journal of Neuroimmunology
|January 11, 2011
PubMed

Antibodies to MOG in serum have a dubious prognostic value in multiple sclerosis. The MOG recombinant protein conformational properties relevant to the antigenic activity are unknown. We employed a solid-phase ELISA based on a product (rMOG(ED)(His)(6)) expressed in E. coli after subcloning the cDNA of the extracellular domain of rat MOG, performing a refolding procedure on column and affinity purification. The far-UV Circular Dichroism (CD) spectra of rMOG(ED)(His)(6) showed a β-sheet, a characteristic feature of the Ig-fold. However, in MS sera and controls we failed to detected IgM or IgG antibodies.

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