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Updated: Jun 5, 2026

Quantitative Detection of Trace Explosive Vapors by Programmed Temperature Desorption Gas Chromatography-Electron Capture Detector
Published on: July 25, 2014
Science based calibration for the extraction of 'analyte-specific' HPLC-DAD chromatograms in environmental analysis
J Kuligowski1, M Martínez Galera, M D Gil García
1Department of Analytical Chemistry, Universidad de Valencia, Edificio Jerónimo Muñoz, 50th, Dr. Moliner, E-46100 Burjassot, Spain.
Abstract:
Multivariate science based calibration (SBC) has been applied to the resolution of overlapped peaks in liquid chromatography with diode array detection (LC-DAD). Complex river water samples spiked with 11 pharmaceutical substances resulted in poorly resolved chromatograms containing additional peaks from interfering matrix compounds and a change in the background absorbance due to the mobile phase gradient. Applying the present multivariate approach it was possible to resolve all 11 analytes from overlapping peaks, obtaining linear calibration lines (R(2)>0.96). Recovery percentages on spiked samples ranged between 74.6 and 113.5%, which are quite satisfactory taking into account the low concentration ranges considered to 1-7 μg L(-1).
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