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High efficiency electroporation of Pseudomonas aeruginosa using frozen cell suspensions
1Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada.
FEMS Microbiology Letters
|July 1, 1990
Abstract:
High efficiency transformation of Pseudomonas aeruginosa was achieved using frozen cell suspensions and high voltage electroporation. We have obtained frequencies as high as 5.8 x 10(8) transformants/micrograms of plasmid DNA using PA01 strain OT684 and a buffer of 15% glycerol-1 mM MOPS. The method allows for easy and reproducible production of frozen cell suspensions for rapid transformation of P. aeruginosa.