Expression and nucleotide sequence analysis of the Legionella pneumophila recA gene

The nucleotide sequence of the L pneumophila recA gene was determined. The coding region was 1044 nucleotides (348 codons), specifying a 37,934 Da protein. Preceding the recA gene was a tandem set of transcription regulatory sequences and putative LexA binding sites. When expressed in E. coli, the cloned recA gene yield two proteins with molecular weights of approximately 38,000 and 35,500 Da. The larger of these two proteins shared 70.4% and 74.6% identity with the E. coli and Pseudomonas aeruginosa RecA proteins, respectively. The 35,500 Da recA encoded protein was presumed to be the product of translation from Met26 which was preceded by an alternate ribosomal binding site.