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Related Concept Videos

Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Meiosis II02:02

Meiosis II

Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
Positive Regulator Molecules01:45

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
Histone Variants at the Centromere02:30

Histone Variants at the Centromere

Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3 variants are also...

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Related Experiment Video

Updated: Jun 5, 2026

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

Centrosomal localization of cyclins E and A: structural similarities and functional differences.

Gaetan Pascreau1, Mair E A Churchill, James L Maller

  • 1Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO, USA.

Cell Cycle (Georgetown, Tex.)
|January 11, 2011
PubMed
Summary

Cyclins A and E

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Related Experiment Videos

Last Updated: Jun 5, 2026

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Cyclins A and E's CLS motifs are crucial for centrosome localization, linking nuclear and centrosomal cell cycles.
  • Proper localization of G1/S cyclins on centrosomes is essential for nuclear DNA replication.
  • DNA replication factors on centrosomes prevent centrosome overduplication, a process dependent on intact CLS motifs.

Purpose of the Study:

  • To compare the structural and functional aspects of cyclin A and E CLS motifs.
  • To identify a novel cyclin A CLS mutant affecting CLS functions and Cdk2 binding.
  • To analyze CLS motif interactions and their role in Cdk2 binding.

Main Methods:

  • Comparative analysis of cyclin A and E CLS motifs.
  • Identification and characterization of a cyclin A CLS mutant.
  • Investigation of CLS motif interactions within cyclin molecules.
  • Assessment of cyclin A-Cdk2 binding affinity.

Main Results:

  • Cyclins A and E share conserved CLS motifs essential for centrosome localization and cell cycle regulation.
  • A novel cyclin A CLS mutant was identified, exhibiting impaired CLS functions and reduced Cdk2 binding.
  • The CBOX1 region of cyclins is critical for their interaction with Cdk2.

Conclusions:

  • The CLS motif is a key regulator of centrosome localization for cyclins A and E, integrating nuclear and centrosomal events.
  • The identified cyclin A CLS mutant provides a tool to further dissect the CLS function in cell cycle control.
  • Understanding CLS-Cdk2 interactions, particularly the role of CBOX1, is vital for comprehending cell cycle regulation and preventing errors like centrosome amplification.