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Published on: January 21, 2018
Biomarkers predicting isocyanate-induced asthma
Nami Shrestha Palikhe1, Joo-Hee Kim, Hae-Sim Park
1Department of Allergy & Rheumatology, Ajou University School of Medicine, Suwon, Korea.
Allergy, Asthma & Immunology Research
|January 11, 2011
Summary
Researchers investigated biomarkers for occupational asthma caused by diisocyanates like TDI, MDI, and HDI. While some markers like serum IgG to tTG and specific HLA haplotypes showed promise, further development is needed for early diagnosis of isocyanate-induced asthma.
Area of Science:
- Occupational medicine
- Immunology
- Genetics
Background:
- Occupational asthma (OA) is a significant health concern linked to diisocyanate exposure.
- Current diagnostic methods for isocyanate-induced OA lack serological testing, necessitating biomarker research.
- Toluene diisocyanate (TDI), 4,4 diphenylmethane diisocyanate (MDI), and 1,6-hexamethylene diisocyanate (HDI) are key causative agents.
Purpose of the Study:
- To identify and evaluate potential immunologic, genetic, neurogenic, and protein biomarkers for diagnosing isocyanate-induced OA.
- To explore novel serological markers using proteomic approaches.
- To investigate genetic associations and neurogenic factors in TDI-induced OA.
Main Methods:
- Analysis of serum IgG antibodies to cytokeratin (CK)18, CK19, and tissue transglutaminase (tTG).
- Investigation of human leukocyte antigen (HLA) and catenin alpha 3 (CTNNA3) genetic polymorphisms.
- Examination of neurokinin 2 receptor (NK2R) polymorphisms and vascular endothelial growth factor (VEGF) levels.
- Proteomic analysis to identify differentially expressed proteins (ferritin, transferrin) in MDI-OA.
- Measurement of serum matrix metalloproteinase-9 (MMP-9) levels.
Main Results:
- Elevated serum IgG to tTG was observed in TDI-OA patients compared to controls.
- Specific HLA haplotype (DRB1*1501-DQB1*0602-DPB1*0501) and CTNNA3 polymorphisms were associated with TDI-OA in Koreans.
- NK2R polymorphisms correlated with VEGF levels in TDI-exposed workers.
- Combined ferritin and transferrin levels showed 71.4% sensitivity and 85.7% specificity for MDI-OA.
- MMP-9 levels were identified as a potential biomarker for TDI-OA.
Conclusions:
- Serum IgG to tTG, specific HLA and CTNNA3 polymorphisms, and MMP-9 show potential as biomarkers for isocyanate-induced OA.
- Ferritin and transferrin levels may aid in diagnosing MDI-OA.
- Further research is crucial for developing comprehensive early diagnostic biomarkers for isocyanate-induced OA.
Keywords:
4,4-diphenylmethane diisocyanateIsocyanatebiomarkersoccupational asthmatoluene diisocyanateMore Related Videos
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