Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genome Annotation and Assembly03:36

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
X-ray Imaging01:24

X-ray Imaging

German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with X-rays, and by 1900, X-ray was widely...
Bone Markings01:26

Bone Markings

Bones have various surface features that help form joints and attach to other soft tissues. Depending on the function, bone markings are categorized into articulating projections, processes for attachment, depressions, and openings.
Articulating Projections
Articulating projections are found where two bones meet to form a joint. These structures are usually found at the ends of bones. The largest articulation is a rounded projection called the head, supported by a narrow neck at the ends of...
EDTA: Auxiliary Complexing Reagents01:26

EDTA: Auxiliary Complexing Reagents

EDTA titrations are usually carried out in highly basic conditions, where the fully deprotonated form of EDTA, Y4−, actively complexes with the free metal ions in the solution. Several metal ions precipitate as hydrous oxide (hydroxides, oxides, or oxyhydroxides) under these conditions, lowering the concentration of free metal ions in the solution. For this reason, auxiliary complexing agents or ligands such as ammonia, tartrate, citrate, or triethanolamine are used in EDTA titrations to...
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single cell RNA sequencing of AML initiating cells reveals RNA-based evolution during disease progression.

Leukemia·2021
Same author

Faricimab: Two in the Bush Is Proving Better than One in the Hand?

Ocular immunology and inflammation·2021
Same author

Peripheral Gangrene in a Rare Scleroderma Overlap Syndrome.

The Journal of the Association of Physicians of India·2021
Same author

Catatonia associated with seizures due to parietal cavernoma.

Indian journal of psychiatry·2021
Same author

<i>TilGAN</i>: GAN for Facilitating Tumor-Infiltrating Lymphocyte Pathology Image Synthesis With Improved Image Classification.

IEEE access : practical innovations, open solutions·2021
Same author

Efficacy of procalcitonin and pentraxin-3 as early biomarkers for differential diagnosis of pleural effusions.

Pleura and peritoneum·2021

Related Experiment Video

Updated: Jun 5, 2026

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment
13:01

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment

Published on: June 3, 2022

Managing and Querying Image Annotation and Markup in XML.

Fusheng Wang1, Tony Pan, Ashish Sharma

  • 1Center for Comprehensive Informatics, Emory University, Atlanta, Georgia, USA.

Proceedings of Spie--The International Society for Optical Engineering
|January 11, 2011
PubMed
Summary

Proprietary data formats hinder image sharing. The Annotation and Image Markup (AIM) project developed a standard model and native XML/XQuery approach for efficient data management and sharing in healthcare research.

More Related Videos

Measuring the Structure, Composition, and Change of Underwater Environments with Large-area Imaging
09:19

Measuring the Structure, Composition, and Change of Underwater Environments with Large-area Imaging

Published on: April 18, 2025

Multiplex Immunohistochemical Analysis of the Spatial Immune Cell Landscape of the Tumor Microenvironment
06:32

Multiplex Immunohistochemical Analysis of the Spatial Immune Cell Landscape of the Tumor Microenvironment

Published on: August 18, 2023

Related Experiment Videos

Last Updated: Jun 5, 2026

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment
13:01

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment

Published on: June 3, 2022

Measuring the Structure, Composition, and Change of Underwater Environments with Large-area Imaging
09:19

Measuring the Structure, Composition, and Change of Underwater Environments with Large-area Imaging

Published on: April 18, 2025

Multiplex Immunohistochemical Analysis of the Spatial Immune Cell Landscape of the Tumor Microenvironment
06:32

Multiplex Immunohistochemical Analysis of the Spatial Immune Cell Landscape of the Tumor Microenvironment

Published on: August 18, 2023

Area of Science:

  • Biomedical Informatics
  • Data Management
  • Medical Imaging

Background:

  • Proprietary data formats create significant barriers for researchers seeking to share image data and knowledge.
  • Standardized approaches are needed for image annotation and markup, especially in healthcare and clinical trial settings.

Purpose of the Study:

  • To address challenges in managing complex hierarchical AIM data.
  • To develop a native XML approach for AIM data management.
  • To support complex image and annotation queries using extended XQuery.

Main Methods:

  • Developed a native XML information model for AIM data.
  • Extended the XQuery language to support complex queries on AIM data.
  • Integrated AIM databases with xService for data sharing via caGrid.

Main Results:

  • Successfully managed complex hierarchical AIM data using a native XML approach.
  • Enabled efficient and complex querying of image and annotation data through native XQuery extensions.
  • Facilitated convenient sharing of AIM databases through caGrid integration.

Conclusions:

  • The native XML and extended XQuery approach effectively manages complex AIM data.
  • This methodology enhances data sharing and querying capabilities in healthcare research environments.
  • Integration with xService and caGrid streamlines the dissemination of clinical image data.