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Updated: Jun 5, 2026

Light-driven Enzymatic Decarboxylation
Published on: May 22, 2016
[Substrate specificity of carotenoid 3',4'-desaturase from Deinococcus radiodurans]
Zongtao Sun1, Bing Tian, Shaochuan Shen
1Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou 310029, China.
Abstract:
To examine the substrate specificity of carotenoid 3',4'-desaturase (DR2250) from Deinococcus radiodurans, we amplified the dr2250 gene by using PCR methods. The PCR products were digested by Hind III-BamH I and ligated into the vector pUC19, yielding recombinant vector pUC-CRTD. We analyzed the carotenoids of E. coli transformants containing pACCRT-EBI(Eu) and (or) pRK-CRTC and (or) pUC-CRTD. Our results demonstrated that DR2250 had substrate specificity on the carotenoids with hydroxyl group at C1 (1').
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