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Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts
Published on: March 2, 2017
Piglets produced from in vivo blastocysts vitrified using the Cryologic Vitrification Method (solid surface
L F S Beebe1, E G Bouwman, S M McIlfatrick
1The University of Adelaide, South Australia, Australia.
Theriogenology
|January 12, 2011
Summary
A new porcine embryo cryopreservation method successfully vitrified embryos without liquid nitrogen contact, preventing contamination. This novel approach led to the birth of 26 piglets from vitrified and warmed embryos.
Area of Science:
- Veterinary Science
- Reproductive Biology
- Cryobiology
Background:
- Porcine embryo cryopreservation is crucial for swine reproduction and genetic conservation.
- Traditional methods risk contamination and damage from direct liquid nitrogen exposure.
Purpose of the Study:
- To develop a simple, successful porcine embryo cryopreservation protocol.
- To prevent direct contact between embryos and liquid nitrogen, minimizing contamination risks.
Main Methods:
- Two solid surface vitrification protocols (with and without centrifugation) were compared using the Cryologic Vitrification Method (CVM).
- A sealed outer container was developed to protect vitrified embryos during storage.
- Vitrified blastocysts were assessed for in vitro survival and cell number, and transferred in vivo.
Main Results:
- Both vitrification protocols showed high in vitro survival rates (90% and 94%) and cell numbers.
- The protocol without centrifugation was selected for further use.
- The sealed container did not negatively impact embryo survival.
- In vivo transfer resulted in a 100% pregnancy rate (5/6 recipients) and 26 live piglets.
Conclusions:
- The developed CVM protocol successfully vitrified porcine embryos without liquid nitrogen contact.
- This method offers a safe and effective approach for porcine embryo cryopreservation and storage.
- The use of a sealed container enhances embryo protection during cryopreservation.
