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Injectable Supramolecular Polymer-Nanoparticle Hydrogels for Cell and Drug Delivery Applications

Injectable Supramolecular Polymer-Nanoparticle Hydrogels for Cell and Drug Delivery Applications

Published on: February 7, 2021

Yielding Behavior in Injectable Hydrogels from Telechelic Proteins.

Bradley D Olsen¹, Julia A Kornfield, David A Tirrell

¹California Institute of Technology Division of Chemistry and Chemical Engineering, Pasadena, CA 91125.

|January 12, 2011

Summary

Injectable hydrogels derived from engineered proteins exhibit significant shear thinning for minimally invasive procedures. This allows for easy injection and rapid recovery, preserving cell viability in tissue engineering and drug delivery applications.

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Preparation of Chitosan-based Injectable Hydrogels and Its Application in 3D Cell Culture

Preparation of Chitosan-based Injectable Hydrogels and Its Application in 3D Cell Culture

Published on: September 29, 2017

Area of Science:

Biomaterials Science
Rheology
Tissue Engineering

Background:

Injectable hydrogels are crucial for minimally invasive tissue engineering and drug delivery.
Existing hydrogels face challenges in achieving optimal injectability and rapid structural recovery.

Purpose of the Study:

To develop and characterize a novel injectable hydrogel with superior shear-thinning and self-healing properties.
To investigate the mechanism behind the hydrogel's rheological behavior and its impact on cell survival.

Main Methods:

Recombinant telechelic proteins expressed in E. coli were used to synthesize the hydrogel.
Rheological analysis, including large amplitude oscillatory shear and flow profile measurements, was performed.
Cell viability was assessed after injection through narrow gauge needles.

Main Results:

The novel hydrogel demonstrated shear thinning by three orders of magnitude at large strains.
A shear-banding mechanism was identified as responsible for the sharp yielding transition.
The hydrogel rapidly recovered its elastic strength within seconds post-injection, with over 95% cell survival.

Conclusions:

The engineered hydrogel offers a promising solution for minimally invasive applications due to its excellent injectability and rapid self-healing.
The shear-banding mechanism facilitates high cell viability during injection.
Genetic engineering can tune the hydrogel's mechanical properties without compromising its critical yielding behavior.