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Related Experiment Video

Updated: Jun 5, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

A microfluidic device with fluorimetric detection for intracellular components analysis.

Radosław Kwapiszewski1, Maciej Skolimowski, Karina Ziółkowska

  • 1Department of Microbioanalytics, Institute of Biotechnology, Warsaw University of Technology, Warsaw, Poland. rkwapiszewski@ch.pw.edu.pl

Biomedical Microdevices
|January 12, 2011
PubMed
Summary
This summary is machine-generated.

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A novel microfluidic system integrates cell lysis and enzyme assays to measure β-glucocerebrosidase activity, a key marker for Gaucher

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Cell Biology

Background:

  • β-glucocerebrosidase activity is crucial for Gaucher's disease diagnosis.
  • Current enzyme assays often involve complex sample preparation.
  • Intracellular enzyme analysis can be affected by proteases released during lysis.

Purpose of the Study:

  • To develop an integrated microfluidic system for rapid β-glucocerebrosidase activity determination.
  • To couple cell lysis and fluorescence-based enzyme assays in a single microdevice.
  • To minimize interference from proteases released during cell lysis.

Main Methods:

  • Fabrication of a poly(dimethylsiloxane) microdevice with lysis, micromeander, and detection zones.
  • Utilizing sheath flow for chemical cell lysis.

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  • Employing fluorescence-based detection of 4-methylumbelliferone, a product of β-glucocerebrosidase activity.
  • Adapting the assay for flow measurements and calculating enzyme activity per cell.

    • Main Results:

    • The microfluidic system successfully determined β-glucocerebrosidase activity in L929 fibroblasts and A549 epithelial cells.
    • Enzyme assays were performed immediately after cell lysis, reducing protease interference.
    • Results showed good correlation with traditional cuvette-based measurements.

    Conclusions:

    • The developed microfluidic system offers an efficient and integrated approach for lysosomal enzyme activity measurement.
    • This method provides a sensitive and reliable alternative for Gaucher's disease biomarker analysis.
    • The system's design minimizes pre-analytical steps and potential enzyme degradation.