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Updated: Jun 5, 2026

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter
Published on: May 21, 2021
Phosphorylcholine (PC)-bonded protein A affinity chromatographic medium for high-throughput purification with reduced
Katsuyuki Maeno1, Aya Hirayama, Kenichi Sakuma
1Shiseido Research Center, 2-2-1 Hayabuchi, Tsuzuki, Yokohama 224-8558, Japan. zhanglantong@263.net
Abstract:
A protein A affinity chromatographic medium based on porous silica modified with phosphorylcholine (PC) groups and amino groups (PNSP) was synthesized. The PC groups functioned as suppressors of non-specific protein adsorption. Recombinant protein A was bound to the amino groups on PNSP with a glutaraldehyde used as a spacer (PNSP-PA). The PC groups and amino groups were immobilized on porous-silica particles using two silane coupling reagents, PC-bound silane, and 3-aminopropyltrimethoxysilane. After optimizing various factors in the synthetic process, the resultant protein A medium showed improvements in non-specific protein adsorption, dynamic binding capacity, and chemical stability under basic conditions compared with conventional protein A affinity media.
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