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Published on: October 7, 2025
[Detection, verification and significance of differentially expressed miRNAs in bladder urothelial carcinoma]
1Department of Urology, Nanjing General Hospital of Nanjing Military Command, Nanjing 210002, China.
Objective:
To explore the differentially expressed miRNAs in bladder urothelial carcinoma (BUC) in order to provide rationales for further studies of function and mechanism for their actions.
Methods:
Fresh normal (5 samples) and BUC (30 samples) tissues were collected from September 2008 to September 2009 in our hospital and analyzed by miRNA microarray to find differentially expressed miRNAs; RT-PCR (reverse transcription-polymerase chain reaction) of recollected samples and 4 types of cell strain to verify the selected miRNAs.
Results:
In grade I, grade II, grade III, grade I + II + III, infiltrating and non-infiltrating groups, hsa-miR-29b-1 was up-regulated while hsa-miR-923 and hsa-miR-300 were down-regulated. When the infiltrating BUC group was compared with the non-infiltrating BUC group, there were 7 differentially expressed miRNAs. In collected samples, the result of RT-PCR was consistent with that of miRNA array. In 4 types of cancer cell strains, only the result of T24 was completely consistent with that of miRNA array.
Conclusions:
There are differentially expressed miRNAs between normal bladder mucosa and BUC, infiltration BUC and non-infiltration BUC. The result of T24 cell strain is completely consistent with that of miRNA array.
