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The diversity and molecular modelling analysis of B₁₂ and B₁₂-independent glycerol dehydratases
Yuemin Liu1, August A Gallo, Rakesh K Bajpai
1Department of Chemistry, University of Louisiana at Lafayette, Lafayette 70504, USA. yx13987@louisiana.edu
Abstract:
To broaden our knowledge on the diversity of glycerol dehydratases, comprehensive sequence and molecular modelling analyses of these enzymes were performed. Our sequence analysis showed that B₁₂-dependent and B₁₂-independent glycerol dehydratases are not related, suggesting that they evolved from different ancestors. Second, our study demonstrated that a gene fusion event occurred between α and β subunits of B₁₂-dependent glycerol dehydratases in several bacteria during enzyme evolution. In addition, our sequence and molecular modelling analyses revealed more B₁₂-independent glycerol dehydratases including hypothetical proteins. Furthermore, we found that some microorganisms contain both B₁₂-dependent and B₁₂-independent glycerol dehydratases in their genomes.
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