The evolution of ferredoxins

Ferredoxins are electron carrier proteins that contain active sites consisting of nonheme iron and inorganic sulfur. They are ubiquitous in living cells and are believed to be among the earliest redox proteins having appeared in primitive organisms. The small size of Ferredoxins allows their amino acid sequences to be determined with relative ease, and nearly a hundred primary structures have been elucidated over the past two decades. Most of these proteins belong to two distinct groups which have been used to construct phylogenetic trees of bacteria and oxygenic photosynthetic organisms respectively. A number of other Ferredoxins, however, seem to be unrelated to any of these two families of proteins and thus raise the problem of the origin of ferredoxins: are they all derived from a common ancestor, or have they appeared and evolved independently several times in the course of biological evolution? This issue is critical in view of the importance of Ferredoxins as evolutionary markers. There is evidence suggesting that presently known ferredoxins belong to at least five independent phyletic lines.