Related Experiment Video
Updated: Jun 5, 2026
Rapid Molecular Detection and Differentiation of Influenza Viruses A and B
Published on: January 30, 2017
Evaluation of serological diagnostic methods for the 2009 pandemic influenza A (H1N1) virus
Jesse Papenburg1, Mariana Baz, Marie-Ève Hamelin
1Infectious Disease Research Center of the CHUQ-CHUL and Laval University, Quebec City, Quebec, Canada.
Abstract:
Serology improves influenza diagnosis by capturing cases missed by reverse transcriptase PCR (RT-PCR). We prospectively evaluated microneutralization and hemagglutination inhibition assays for 2009 influenza A (H1N1) virus diagnosis among 24 RT-PCR-confirmed cases and 98 household contacts. Compared to hemagglutination inhibition, microneutralization demonstrated a higher level of concordance with RT-PCR (kappa = 0.69 versus kappa = 0.60) and greater sensitivity (83% versus 71%; P = 0.016).
