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Five carboxin-resistant mutants exhibited various responses to carboxin and related fungicides

Yoko Shima1, Yasuhiro Ito, Hidemi Hatabayashi

  • 1Food Biotechnology Division, National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.

Bioscience, Biotechnology, and Biochemistry
|January 14, 2011
PubMed

Insights

Five Aspergillus oryzae mutants resistant to carboxin showed distinct sensitivities to related fungicides. This research offers insights into the molecular interactions between succinate dehydrogenase (SDH) and these agricultural fungicides.

Area of Science:

  • Biochemistry
  • Mycology
  • Agricultural Science

Background:

  • Fungicides are crucial for crop protection.
  • Succinate dehydrogenase (SDH) is a key enzyme in fungal respiration and a target for fungicides like carboxin.
  • Understanding fungicide resistance mechanisms is vital for sustainable agriculture.

Purpose of the Study:

  • To characterize carboxin-resistant mutants of Aspergillus oryzae.
  • To investigate the differential sensitivity of these mutants to carboxin and related fungicides.
  • To elucidate the molecular interactions between SDH and fungicides.

Main Methods:

  • Isolation and characterization of five carboxin-resistant Aspergillus oryzae mutants.
  • Assessing mycelial growth sensitivity to fungicides.
  • Measuring succinate dehydrogenase (SDH) activity in the presence of fungicides.

Main Results:

  • All five mutants exhibited significant resistance to carboxin.
  • Each mutant displayed unique sensitivity patterns to the other three tested fungicides.
  • Differential sensitivity suggests distinct molecular interactions with SDH.

Conclusions:

  • Carboxin resistance in Aspergillus oryzae can arise through various mechanisms.
  • Fungicide cross-resistance is not uniform, indicating specific molecular targets.
  • These findings provide valuable information for understanding SDH-fungicide interactions and developing new fungicides.

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