A modified method for consistent and reliable Golgi-cox staining in significantly reduced time
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Updated: Jun 5, 2026

Assessment of Hippocampal Dendritic Complexity in Aged Mice Using the Golgi-Cox Method
Published on: June 22, 2017
Amit Ranjan1, Birendra Nath Mallick
1School of Life Sciences, Jawaharlal Nehru University New Delhi, India.
Abstract:
The two major limitations of Golgi-Cox method are that staining takes very long time and it is inconsistent. In this paper we describe a modification of the Golgi-Cox method, in which the tissue blocks were maintained at 37 ± 1°C during chromation for only 24 h and consistent staining of neurons in rat brain sections were observed. The method is simple, reproducible, rapid, inexpensive, and provides uniform staining with very good resolution of neuronal soma, dendrites as well as spines.
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