Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

X-ray Imaging

X-ray Imaging

German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with X-rays, and by 1900, X-ray was widely...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Quantum Stroboscopy for Time Measurements.

Physical review letters·2026

Same author

Erratum: Quantum Radar [Phys. Rev. Lett. 124, 200503 (2020)].

Physical review letters·2023

Same author

Non-line-of-sight snapshots and background mapping with an active corner camera.

Nature communications·2023

Same author

Quantum Metrology of Noisy Spreading Channels.

Physical review letters·2022

Same author

Spectral control of nonclassical light pulses using an integrated thin-film lithium niobate modulator.

Light, science & applications·2022

Same author

Surface Plasmon Enhanced Upconversion Fluorescence in Short-Wave Infrared for In Vivo Imaging of Ovarian Cancer.

ACS nano·2022

Search research articles

Related Experiment Video

Updated: Jun 5, 2026

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

Sub-Rayleigh imaging via N-photon detection.

Fabrizio Guerrieri¹, Lorenzo Maccone, Franco N C Wong

¹Dipartimento di Elettronica e Informazione, Politecnico di Milano, 20133 Milano, Italy.

Physical Review Letters

|January 15, 2011

Summary

This summary is machine-generated.

Researchers achieved sub-Rayleigh resolution using N-photon photodetection and a scanning focused beam. This novel imaging technique surpasses conventional limits for distinguishing point objects.

More Related Videos

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

Related Experiment Videos

Last Updated: Jun 5, 2026

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

Area of Science:

Optics and Photonics
Quantum Imaging

Background:

The Rayleigh diffraction limit restricts the resolution of conventional imaging systems.
Distinguishing closely spaced objects is a fundamental challenge in optics.

Purpose of the Study:

To demonstrate imaging resolution beyond the classical Rayleigh diffraction bound.
To explore the application of N-photon photodetection for enhanced resolution.

Main Methods:

Utilizing a scanning focused beam in an arbitrary pattern.
Implementing N-photon photodetection with a single-photon avalanche detector array.
Analyzing resolution improvement based on detected photon numbers.

Main Results:

Achieved sub-Rayleigh resolution, surpassing the classical limit.
Demonstrated a resolution improvement factor of approximately (N-N(max))(½).
Experimental results align well with theoretical predictions.

Conclusions:

N-photon photodetection combined with scanning offers a pathway to overcome classical resolution limits.
This technique provides significant resolution enhancement for imaging point objects.