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Updated: Jun 5, 2026

Passaging Human Neural Stem Cells

Passaging Human Neural Stem Cells

Published on: August 22, 2007

Human chorion-derived stem cells: changes in stem cell properties during serial passage.

Mohd-Manzor Nur Fariha¹, Kien-Hui Chua, Geok-Chin Tan

¹Department of Pathology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.

|January 15, 2011

Summary

Human chorion-derived stem cells (hCDSC) demonstrate robust stemness properties and multipotent differentiation potential. These cells maintain chromosomal stability through serial passaging, indicating their promise for future cell therapy applications.

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Comparison of Two Representative Methods for Differentiation of Human Induced Pluripotent Stem Cells into Mesenchymal Stromal Cells

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Published on: October 20, 2023

Area of Science:

Stem Cell Biology
Regenerative Medicine
Biotechnology

Background:

Fetal membranes from human placenta are recognized as a source of stem cells.
While amnion stem cells are well-studied, chorion-derived stem cells (hCDSC) remain less explored.
Limited research exists on the specific stem cell characteristics of chorion-derived cells.

Purpose of the Study:

To investigate and confirm the stemness properties of serially passaged human chorion-derived stem cells (hCDSC).
To assess the gene expression profile related to stemness in hCDSC.
To evaluate the differentiation potential and chromosomal stability of hCDSC.

Main Methods:

Quantitative PCR was used to analyze stemness gene expression (Oct-4, Sox-2, Nanog, etc.) in serial-passaged hCDSC.
Cell growth rate, colony-forming unit-fibroblast (CFU-F) frequency, and multilineage differentiation (adipogenic, chondrogenic, osteogenic) were assessed.
Immunophenotyping for surface markers, immunostaining for trophoblast markers, and cytogenetic analysis for chromosomal stability were performed.

Main Results:

Mesenchymal lineage markers and stemness genes (except TERT) were highly expressed in hCDSC, increasing with serial passaging.
hCDSC exhibited high clonogenic potential (1:30 CFU-F frequency) and successful multilineage differentiation.
Cytogenetic analysis confirmed chromosomal stability in hCDSC after serial passaging, with no detectable trophoblast contamination.

Conclusions:

Human chorion-derived stem cells (hCDSC) possess significant stemness and multipotent differentiation capabilities.
hCDSC maintain their therapeutic potential even after multiple passages.
These findings position hCDSC as a promising candidate for future cell-based therapies.