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Updated: Jun 5, 2026

Organotypic Cerebellar Cultures: Apoptotic Challenges and Detection
11:57

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Published on: May 17, 2011

Neuronal apoptosis in the developing cerebellum.

X-S Cheng1, M-S Li, J Du

  • 1Institute of Neurobiology, Medical College of Henan University, Kaifeng 475004, Henan Province, China.

Anatomia, Histologia, Embryologia
|January 15, 2011
PubMed
Summary
This summary is machine-generated.

This study investigated programmed cell death (apoptosis) in developing mouse cerebellum. Key apoptotic activity occurs between postnatal days 0-14, suggesting a critical window for treating neurodegenerative diseases.

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Cell Biology

Background:

  • Neuronal development involves programmed cell death (apoptosis).
  • Understanding apoptosis timing in the cerebellum is crucial for developmental neuroscience.
  • The developing cerebellum is a key area for motor control and cognitive functions.

Purpose of the Study:

  • To analyze the temporal dynamics of apoptosis in specific cerebellar layers during development.
  • To identify the key developmental period for neuronal apoptosis in the mouse cerebellum.
  • To explore potential therapeutic windows for congenital neurodegenerative diseases.

Main Methods:

  • Immunohistochemistry for active caspase-3.
  • Hoechst 33258 staining for cell nuclei.
  • Western blot analysis for caspase-3 and caspase-8 expression.

Main Results:

  • Apoptosis peaked at different postnatal days (P5-P9) across cerebellar layers (EGL, PCL, IGL).
  • Caspase-3 expression peaked at P5 and declined by P14; caspase-8 peaked at P10 and disappeared by P30.
  • Apoptotic rates decreased with age in all analyzed regions.

Conclusions:

  • The critical period for cerebellar neuronal apoptosis is between postnatal days 0 and 14.
  • This developmental window presents a potential target for therapeutic interventions in congenital neurodegenerative disorders.
  • Caspase activity patterns correlate with observed apoptosis timing in the developing cerebellum.