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Updated: Apr 28, 2026
Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix
Published on: February 9, 2015
Culture and identification of human amniotic mesenchymal stem cells
Huo Shuang-zhi1, Shi Ping, Pang Xi-ning
1Department of Stem Cells and Regenerative Medicine, China Medical University, Shenyang 110001, China.
Objective:
To establish the method of isolation, purification, and identification of human amniotic mesenchymal stem cells (hAMSCs).
Methods:
hAMSCs were isolated from human amniotic membrane by trypsin-collagenase digestion, and cultured in Dulbecco's modified Eagle's medium/F12 medium supplemented with 10% fetal bovine serum. Phenotypic characteristics of these cells were analyzed by means of immunocytochemistry and flow cytometry.
Results:
The cells successfully isolated from human amniotic membrane expressed representative mesenchymal cell surface markers CD44, CD90, and vimentin, but not CD45.
Conclusions:
This study establishes a potential method for isolation of hAMSCs from human amnion,in vitro culture, and identification. The isolated cells show phenotypic characteristics of mesenchymal stem cells.
