Related Experiment Video
Updated: Jun 5, 2026
Pre-Conditioning the Airways of Mice with Bleomycin Increases the Efficiency of Orthotopic Lung Cancer Cell Engraftment
Published on: June 28, 2018
[Establishment of a human large cell lung cancer cell line L9981-nm23-H1]
Liangan Che1, Qinghua Zhou, Yanping Wang
1The Key Laboratory of Lung Cancer Molecular Biology of Sichuan Province and Department of Thoracic Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R.China.
Background:
To establish a human large cell lung cancer cell line L9981-nm23-H1 transfected with wild type nm23-H1 gene.
Methods:
pLXSN-nm23-H1-EGFP was constructed by gene clone technique, and L9981-nm23-H1 was established by infected virus of nm23-H1 gene. The DNA and protein expression of nm23-H1 were detected in the transgene large cell lung cancer cell line by PCR and Western blot.
Results:
pLXSN-nm23-H1-EGFP was constructed successfully, and the nm23-H1 cDNA was inducted to L9981 cell. The protein of nm23-H1 could be detected in L9981-nm23-H1 cell.
Conclusions:
Protein of nm23-H1 is stably, continuously and high efficiently expressed in L9981-nm23-H1 cell.
