Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Thin-Layer Chromatography (TLC): Overview01:11

Thin-Layer Chromatography (TLC): Overview

Thin-layer chromatography (TLC) is a chromatography technique that separates compounds based on their polarity. TLC typically uses polar silica gel, a form of silicon dioxide, as the stationary phase. The silica gel contains hydroxyl (OH) groups on its surface, which form hydrogen bonds with polar compounds, influencing their adhesion to the stationary phase.
To begin the analysis, a mixture of compounds is spotted on the starting line on the TLC plate using a thin capillary. The bottom of the...
Gas Chromatography: Types of Detectors-I01:21

Gas Chromatography: Types of Detectors-I

There are different types of detectors used in gas chromatography, each with its own specific properties that make it suitable for detecting certain types of analytes. The most commonly used detectors in GC are thermal conductivity detector (TCD), flame ionization detector (FID), and electron capture detector (ECD).
TCD is the earliest and most widely used detector that operates by measuring the changes in the thermal conductivity of the carrier gas. When a sample compound enters the detector,...
High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...
Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rv0100: An essential acyl carrier protein from M. tuberculosis important in dormancy.

PloS one·2024
Same author

Pharmacological inhibition of CLK2 activates YAP by promoting alternative splicing of AMOTL2.

eLife·2023
Same author

Editorial: Medicine and food homology: emerging tool and methodology for separation and analysis of the bioactive factors.

Frontiers in nutrition·2023
Same author

Pharmacological YAP activation promotes regenerative repair of cutaneous wounds.

Proceedings of the National Academy of Sciences of the United States of America·2023
Same author

Pharmacological inhibition of CLK2 activates YAP by promoting alternative splicing of AMOTL2.

bioRxiv : the preprint server for biology·2023
Same author

Effect-Directed Profiling of <i>Akebia quinata</i> and <i>Clitoria ternatea</i> via High-Performance Thin-Layer Chromatography, Planar Assays and High-Resolution Mass Spectrometry.

Molecules (Basel, Switzerland)·2023

Related Experiment Video

Updated: Jun 5, 2026

Bioassay-Guided Identification of Natural Products for Biocontrol by Thin Layer Chromatography-Direct Bioautography
04:52

Bioassay-Guided Identification of Natural Products for Biocontrol by Thin Layer Chromatography-Direct Bioautography

Published on: July 26, 2024

Bioautography detection in thin-layer chromatography.

Irena M Choma1, Edyta M Grzelak

  • 1Department of Chromatographic Methods, University of Maria Curie-Skłodowska, M. Skłodowska Sq. 3, 20-031 Lublin, Poland. irena.choma@umcs.lublin.pl

Journal of Chromatography. A
|January 15, 2011
PubMed
Summary
This summary is machine-generated.

Bioautography, a microbial detection method, uses antimicrobial properties to analyze compounds. This review focuses on direct bioautography and related techniques like TLC-bioluminescence screening for compound analysis and bacterial growth studies.

More Related Videos

Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds
12:04

Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds

Published on: March 27, 2014

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
13:57

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects

Published on: February 18, 2014

Related Experiment Videos

Last Updated: Jun 5, 2026

Bioassay-Guided Identification of Natural Products for Biocontrol by Thin Layer Chromatography-Direct Bioautography
04:52

Bioassay-Guided Identification of Natural Products for Biocontrol by Thin Layer Chromatography-Direct Bioautography

Published on: July 26, 2024

Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds
12:04

Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds

Published on: March 27, 2014

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
13:57

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects

Published on: February 18, 2014

Area of Science:

  • Microbiology
  • Analytical Chemistry
  • Biochemistry

Background:

  • Bioautography is a powerful technique for detecting antimicrobial and antifungal compounds.
  • It is often coupled with planar chromatography for substance analysis.
  • Understanding microbial interactions and compound properties is crucial in drug discovery.

Purpose of the Study:

  • To review the different versions of bioautography, with a focus on direct bioautography.
  • To highlight the applications of bioautography in analyzing various compounds and biochemical processes.
  • To discuss related methods that enhance direct bioautography, such as TLC-bioluminescence screening.

Main Methods:

  • The review discusses three main types of bioautography: contact, immersion, and direct.
  • Direct bioautography is explored in greater detail.
  • Related techniques, including Thin Layer Chromatography (TLC)-bioluminescence screening, are also considered.

Main Results:

  • Bioautography effectively identifies substances with antimicrobial or antifungal activity.
  • Direct bioautography offers versatile applications for compound screening and studying factors affecting bacterial growth.
  • TLC-bioluminescence screening emerges as a promising related method for microbial detection.

Conclusions:

  • Bioautography, particularly the direct method, is a valuable tool for antimicrobial compound discovery and research.
  • The integration of related techniques can further enhance the capabilities of bioautography.
  • This method aids in understanding complex biochemical processes and microbial behavior.