Related Experiment Video
Updated: Jun 5, 2026

Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram
Published on: November 27, 2016
Reverse migration order of sibutramine enantiomers as a function of cyclodextrin concentration in capillary
Hongmei Zhu1, Enqi Wu, Jianbo Chen
1College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea.
Abstract:
The current study demonstrates the reversal of enantiomer migration order (EMO) in capillary electrophoresis (CE) based separations of sibutramines (SIB) as a function of the concentration of two types of cyclodextrin (CD), native β-CD and acetyl-β-CD. At normal working concentrations (<10mM) of either CD, (S)-SIB migrated first. However, at CD concentrations greater than 10mM, (R)-SIB was the first to migrate. This study describes factors involved in determining EMO for sibutramine enantiomers at low and high concentrations of CDs. The reversal of EMO could be explained in terms of the opposing effects of the stability and the limiting complex mobility of the SIB-CD complexes. The enantioseparation of SIB with methyl- and 2-hydroxypropyl-β-CD was possible based on differences in the binding constants of complexes. However, reverse EMO was not observed because of equal mobilities of SIB enantiomers complexed with methyl- and 2-hydroxypropyl-β-CD.
Related Concept Videos
Racemic Mixtures and the Resolution of Enantiomers
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Properties of Enantiomers and Optical Activity
Naming Enantiomers
Analyte Adsorption and Distribution
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...

