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Related Experiment Video

Updated: Jun 5, 2026

Application of Electrophysiology Measurement to Study the Activity of Electro-Neutral Transporters
11:51

Application of Electrophysiology Measurement to Study the Activity of Electro-Neutral Transporters

Published on: February 3, 2018

Ectonucleotidases in the digestive system: focus on NTPDase3 localization.

Elise G Lavoie1, Brian D Gulbransen, Mireia Martín-Satué

  • 1Centre de Recherche en Rhumatologie et Immunologie, Centre Hospitalier Universitaire de Québec, QC, Canada.

American Journal of Physiology. Gastrointestinal and Liver Physiology
|January 15, 2011
PubMed
Summary

This study maps the distribution of ectonucleotidases, including NTPDase3, in the digestive system. Findings reveal specific localizations suggesting roles in epithelial secretion and nerve signaling.

Related Experiment Videos

Last Updated: Jun 5, 2026

Application of Electrophysiology Measurement to Study the Activity of Electro-Neutral Transporters
11:51

Application of Electrophysiology Measurement to Study the Activity of Electro-Neutral Transporters

Published on: February 3, 2018

Area of Science:

  • Gastroenterology and Molecular Biology
  • Enzymology and Receptor Signaling

Background:

  • Extracellular nucleotides and adenosine are key signaling molecules regulated by ectonucleotidases.
  • These molecules interact with P1 and P2 receptors, influencing digestive system functions like secretion and neurotransmission.
  • The distribution of ectonucleotidases, particularly NTPDase3, in the digestive tract is largely uncharacterized.

Purpose of the Study:

  • To investigate the localization of ectonucleotidases, focusing on NTPDase3, within the gastrointestinal tract and salivary glands.
  • To elucidate the potential roles of these enzymes in digestive system physiology.

Main Methods:

  • Semiquantitative RT-PCR to assess gene expression.
  • Immunohistochemistry and in situ enzyme activity assays for protein localization and function.
  • Calcium (Ca2+) imaging to study P2 receptor ligand availability in the enteric nervous system.

Main Results:

  • NTPDase3 was identified in epithelial cells of salivary glands, esophagus, forestomach, and gastric antrum, as well as in colonic neurons.
  • NTPDase2 and ecto-5'-nucleotidase were found to be coexpressed with NTPDase3 in salivary glands and stratified epithelia.
  • NTPDases were shown to modulate P2 receptor ligand availability in the enteric nervous system.

Conclusions:

  • The specific localization of NTPDase3 suggests critical roles in digestive epithelial functions, including secretion.
  • Coexpression with NTPDase2 and ecto-5'-nucleotidase indicates coordinated functions in the digestive system.
  • These ectonucleotidases are important regulators of enteric neurotransmission and P2 receptor signaling.