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Updated: Jun 5, 2026

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
Published on: December 29, 2015
Full genome sequence and virulence analyses of the recent equine isolate of Japanese encephalitis virus
Masayuki Shimojima1, Yumiko Nagao, Hiroshi Shimoda
1Laboratory of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University, Japan.
Abstract:
In the past 25 years, there has been only one case of Japanese encephalitis in horses in Japan. We determined the full genome sequence of the Japanese encephalitis virus (JEV) strain JEV/eq/Tottori/2003 isolated from an afflicted horse and also analyzed its virulence in mice. The sequence analysis showed that the genome of JEV/eq/Tottori/2003 is similar to that of genotype I, a dominant genotype of JEV presently circulating in Japan. Its neurovirulence, but not neuroinvasiveness, was still as high as it was for genotype III, thus indicating the necessity for continuation of a vaccination program of horses against JEV.
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