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Updated: Aug 14, 2026
Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α
Published on: June 14, 2018
[Enzyme immunoassays for the quantification of human gamma-gamma-enolase (NSE)]
J Gross1, J Zinsmeyer, R Pohle
1Institut für Pathologische und Klinische Biochemie, Bereichs Medizin (Charité) der Humboldt-Universität zu Berlin.
Abstract:
A direct two-site binding assay on the basis of antibodies from sheep for the quantification of human gamma-gamma enolase is described. The antibody was produced by immunization with human NSE coupled to horse spleen ferritin. The assay shows two feature: a decreased reactivity with NSE from rat and NSE from human serum in spite of 100% recovery of purified human brain NSE. The sheep antibody seems to react with epitopes less accessible on the rat NSE and on the NSE of human serum. The assay is characterized by gamma-gamma enolase specificity, a high sensitivity (2 pg) and a precision of CV = 3-7%.
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