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Related Experiment Videos

Efficient transformation of chicken embryo fibroblasts by c-Jun requires structural modification in coding and

T J Bos1, F S Monteclaro, F Mitsunobu

  • 1Department of Microbiology, University of Southern California, School of Medicine, Los Angeles 90033.

Genes & Development
|October 1, 1990
PubMed
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The oncogenic potential of Jun proteins was investigated. Deleting specific amino acid regions and 3’ untranslated sequences from c-Jun significantly enhanced its cell transformation ability, similar to v-Jun.

Area of Science:

  • Oncogenic transformation
  • Molecular biology
  • Retroviral gene expression

Background:

  • The c-Jun protein is a proto-oncogene implicated in cell proliferation and transformation.
  • Avian retroviral vectors like RCAS are used to deliver and express genes in avian cells.
  • Understanding the structural differences between cellular (c-Jun) and viral (v-Jun) oncogenes is crucial for elucidating oncogenic mechanisms.

Purpose of the Study:

  • To evaluate the transforming capability of the c-Jun protein.
  • To compare the oncogenic potential of c-Jun with v-Jun.
  • To identify specific structural features of Jun that contribute to its oncogenic activity.

Main Methods:

  • Introduction of chicken c-jun proto-oncogene into a replication-competent avian retroviral expression vector (RCAS).

Related Experiment Videos

  • Expression of viral Jun (v-Jun) and c-Jun in chicken embryo fibroblasts (CEFs).
  • Construction and analysis of deletion and substitution mutants of c-Jun to assess oncogenic activity via focus formation assays.

    • Main Results:

    • Viral Jun (v-Jun) efficiently transformed chicken embryo fibroblasts (CEFs).
    • Overexpression of c-Jun transformed CEFs less efficiently than v-Jun (15- to 25-fold lower).
    • Deletion of a 27-amino acid region near the N-terminus of c-Jun and deletion of 3'-untranslated sequences were critical for activating full oncogenic potential.

    Conclusions:

    • Structural differences between c-Jun and v-Jun significantly impact oncogenic potential.
    • Specific regions within c-Jun, particularly an N-terminal segment and 3'-untranslated sequences, are key determinants of its transforming ability.
    • Modifications to c-Jun can enhance its oncogenic activity, providing insights into viral oncogene activation.