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Purification of pertussis toxin substrates from bovine heart membranes
K Urasawa1, K Sato, T Murakami
1Department of Cardiovascular Medicine, Hokkaido University, Sapporo, Japan.
Life Sciences
|January 1, 1990
Abstract:
Pertussis toxin substrates were purified from bovine heart membranes by means of a sequential column chromatographic techniques. The elution pattern from the final ion-exchange chromatography and relative mobilities of these peptides on SDS-polyacrylamide gel electrophoresis revealed that the bovine heart membranes contained two distinct pertussis toxin substrates: Gi2 and Go.