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Related Experiment Videos

cDNA clone encoding Drosophila transcription factor TFIID.

M L Muhich1, C T Iida, M Horikoshi

  • 1Division of Chemistry, California Institute of Technology, Pasadena 91125.

Proceedings of the National Academy of Sciences of the United States of America
|December 1, 1990
PubMed
Summary
This summary is machine-generated.

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Researchers isolated and characterized a cDNA clone for the Drosophila TFIID protein, essential for transcription initiation. This finding reveals conserved and divergent regions between Drosophila, yeast, and human TFIID, offering insights into transcription regulation.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Transcription initiation by RNA polymerase II is a critical regulatory process.
  • The TATA-binding protein complex TFIID plays a crucial role in this initiation.
  • Understanding TFIID across different species provides insights into conserved and divergent regulatory mechanisms.

Purpose of the Study:

  • To isolate and characterize the cDNA encoding the Drosophila TFIID protein.
  • To compare the structural and sequence homology of Drosophila TFIID with its counterparts in yeast and humans.
  • To identify potential regulatory motifs within the Drosophila TFIID amino-terminal region.

Main Methods:

  • cDNA cloning and sequencing.
  • Deduced amino acid sequence analysis.

Related Experiment Videos

  • Homology comparisons using sequence alignment algorithms.

    • Main Results:

    • A cDNA clone for Drosophila TFIID was successfully isolated and characterized.
    • The deduced amino acid sequence showed significant homology (80-88%) in the carboxyl-terminal region with yeast and human TFIID.
    • The amino-terminal regions showed less homology between Drosophila and yeast, but qualitative similarity between Drosophila and humans.
    • Specific sequence motifs, implicated in transcription regulation, were identified in the Drosophila TFIID amino-terminus.

    Conclusions:

    • The Drosophila TFIID protein shares conserved functional domains with yeast and human TFIID, particularly in the carboxyl terminus.
    • Divergence in the amino-terminal regions suggests species-specific regulatory roles or interactions.
    • The identified motifs in the Drosophila TFIID amino-terminus may contribute to its role in regulating transcription in this organism.