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Mitogen-induced activation of stable E-rosettes by human lymphocytes
Immunological Communications
|January 1, 1978
Summary
Phytohemagglutinin (PHA) activates human lymphocytes to form stable E-rosettes. Other mitogens like Concanavalin A and sodium periodate also activate lymphocytes, but soybean agglutinin does not.
Area of Science:
- Immunology
- Cell Biology
Background:
- Phytohemagglutinin (PHA) is known to activate human lymphocytes.
- Lymphocyte activation can lead to the formation of stable E-rosettes with sheep red blood cells at 37°C.
Purpose of the Study:
- To investigate the ability of various mitogens to activate human lymphocytes.
- To determine if other mitogens can induce stable E-rosette formation.
Main Methods:
- Testing several mitogens: Concanavalin A, pokeweed mitogen, soybean agglutinin, sodium periodate, and galactose oxidase.
- Assessing the capacity of these mitogens to activate human lymphocytes for stable E-rosette formation.
Main Results:
- Concanavalin A, pokeweed mitogen, sodium periodate, and galactose oxidase activated lymphocytes to form stable E-rosettes.
- Soybean agglutinin did not demonstrate this capability.
Conclusions:
- Multiple mitogens, beyond PHA, can induce stable E-rosette formation in human lymphocytes.
- This suggests a broader range of substances can modulate lymphocyte surface interactions.