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Related Concept Videos

High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...
Thin-Layer Chromatography (TLC): Overview01:11

Thin-Layer Chromatography (TLC): Overview

Thin-layer chromatography (TLC) is a chromatography technique that separates compounds based on their polarity. TLC typically uses polar silica gel, a form of silicon dioxide, as the stationary phase. The silica gel contains hydroxyl (OH) groups on its surface, which form hydrogen bonds with polar compounds, influencing their adhesion to the stationary phase.
To begin the analysis, a mixture of compounds is spotted on the starting line on the TLC plate using a thin capillary. The bottom of the...
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
Principles Of Column Chromatography01:13

Principles Of Column Chromatography

The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
Gas Chromatography: Types of Detectors-I01:21

Gas Chromatography: Types of Detectors-I

There are different types of detectors used in gas chromatography, each with its own specific properties that make it suitable for detecting certain types of analytes. The most commonly used detectors in GC are thermal conductivity detector (TCD), flame ionization detector (FID), and electron capture detector (ECD).
TCD is the earliest and most widely used detector that operates by measuring the changes in the thermal conductivity of the carrier gas. When a sample compound enters the detector,...

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Related Experiment Video

Updated: Jun 5, 2026

Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds
12:04

Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds

Published on: March 27, 2014

Thin-layer chromatography with biological detection in phytochemistry.

A Marston1

  • 1Chemistry Department, University of the Free State, Bloemfontein 9300, South Africa. marstona@ufs.ac.za

Journal of Chromatography. A
|January 18, 2011
PubMed
Summary
This summary is machine-generated.

Bioautography on thin-layer chromatography (TLC) plates efficiently detects biological activity in complex mixtures, ideal for isolating plant compounds. This method uses minimal sample and allows for solvent removal before bioassays, compatible with various tests like antimicrobial and antioxidant assays.

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Bioassay-Guided Identification of Natural Products for Biocontrol by Thin Layer Chromatography-Direct Bioautography
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Bioassay-Guided Identification of Natural Products for Biocontrol by Thin Layer Chromatography-Direct Bioautography

Published on: July 26, 2024

Related Experiment Videos

Last Updated: Jun 5, 2026

Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds
12:04

Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds

Published on: March 27, 2014

Bioassay-Guided Identification of Natural Products for Biocontrol by Thin Layer Chromatography-Direct Bioautography
04:52

Bioassay-Guided Identification of Natural Products for Biocontrol by Thin Layer Chromatography-Direct Bioautography

Published on: July 26, 2024

Area of Science:

  • Analytical Chemistry
  • Pharmacognosy
  • Biochemistry

Background:

  • Bioautography on thin-layer chromatography (TLC) plates is a powerful technique for identifying biologically active compounds.
  • It is particularly useful for analyzing complex natural product mixtures, such as plant extracts.

Purpose of the Study:

  • To highlight the utility of TLC bioautography for detecting and isolating bioactive constituents from complex samples.
  • To demonstrate its advantages over other chromatographic methods like High-Performance Liquid Chromatography (HPLC).

Main Methods:

  • Thin-layer chromatography (TLC) coupled with bioassays (bioautography).
  • Solvent removal post-chromatography to ensure compatibility with biological detection systems.
  • Application of various bioassays, including antimicrobial, radical scavenging, antioxidant, and enzyme inhibition tests.

Main Results:

  • TLC bioautography requires minimal sample amounts, making it cost-effective.
  • Multiple samples can be analyzed simultaneously, increasing throughput compared to HPLC.
  • Complete removal of organic solvents ensures the viability of biological detection systems.

Conclusions:

  • TLC bioautography is an efficient, target-directed method for discovering and isolating bioactive compounds from complex mixtures.
  • Its compatibility with diverse bioassays and ability to handle multiple samples make it a versatile tool in natural product research.